Gene machines

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Colorful molecular diagnostics.

Clin Chem 58:4 (2012) 659-660

Authors:

Achillefs N Kapanidis, Robert Crawford

A protein biosensor that relies on bending of single DNA molecules.

Chemphyschem 13:4 (2012) 918-922

Authors:

Robert Crawford, Douglas J Kelly, Achillefs N Kapanidis

Abstract:

A "bendy" protein sensor: A DNA-based sensor that uses folded DNA (through DNA kinks) and protein-induced bending to detect DNA-binding proteins is presented. Single-molecule sensing of a transcriptional activator (catabolite activator protein, CAP, which bends its DNA site by 80掳) is demonstrated in solution and on surfaces, both in buffers and in cell lysates. The method should allow detection of a wide range of DNA-bending proteins.

Recognition of different DNA sequences by a DNA鈥恇inding protein alters protein dynamics differentially

FEBS Letters Wiley 586:3 (2012) 258-262

Authors:

Tanumoy Mondol, Subrata Batabyal, Abhishek Mazumder, Siddhartha Roy, Samir Kumar Pal

Detection of Specific mRNA Synthesis in Rotavirus using Single Molecule Hybridization

BIOPHYSICAL JOURNAL 102:3 (2012) 287A-287A

Authors:

Javier Periz, Cristina Celma, Justin Pinkney, Polly Roy, Achillefs Kapanidis

In vivo single-molecule biochemistry of chromosome replication and segregation in bacteria.

MOLECULAR BIOLOGY OF THE CELL 23 (2012)

Authors:

D Sherratt, R Reyes-Lamothe, A Badrinaryanan, E Nicolas, O Henry, A Kapanidis, S Uphoff, M Leake