2P532 High-resolution dynamic imaging of membrane proteins by high-speed AFM(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Seibutsu Butsuri Biophysical Society of Japan 46:supplement2 (2006) s428
Cell volume increase in murine MC3T3-E1 pre-osteoblasts attaching onto biocompatible tantalum observed by magnetic AC mode atomic force microscopy.
Eur Cell Mater 10 (2005) 61-68
Abstract:
Magnetic AC mode (MACmode) atomic force microscopy (AFM) was used to study murine (mouse) MC3T3-E1 preosteoblastic cells attached to biocompatible tantalum substrates. Cell volumes of attached cells derived from AFM images were compared to volumes of detached cells in suspension measured by the Coulter sizing technique. An increase of approximately 50% in cell volume was observed when the cells attached to planar tantalum substrates and developed a flattened structure including lamellipodia. We address thoroughly the issues general to the AFM determination of absolute cell volumes, and compare our magnetic AC mode AFM measurements to hitherto reported cell volume determinations by contact mode AFM.Unfolding and extraction of a transmembrane alpha-?helical peptide: Dynamic Force Spectroscopy and Molecular Dynamics Simulations
Biophysical Journal 89 (2005) 3129-3140
Characterization of beta-amyloid(1-40) incorporated in model membranes: peptide conformation, acyl chain order, vesicle morphology, supported bilayer topography, and channel activity
FEBS J 272 (2005) 235-236
Bionanotechnology with Membrane Proteins: Mechanics and Electronics
Japan Society of Applied Physics (2005)